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Note: Incubation time will vary depending on cell line and cell density Prepare MTT stock solution 2 Dissolve 5 mg MTT in 1 ml 1X PBS

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mtt stock MTT Stock Solution: Prepare MTT reagent by dissolving 250 mg MTT in 50 mL 1X PBS solution, and Filter it via 0,2 µM filter MTT stock solution can be stored